ABSTRACT
A new subspecies of the genus Streptomyces, the proposed named of which is Streptomyces lipmanii ZAG-37 a soil isolate producing alpha -amylase is described. The mature aerial mass of the organism are in the yellow colour series when grown on the ISP media. Long chains of unbranched sporophores occur in both the aerial and substrate mycelia. The spore surface are smooth. LL-Diaminopimelic acid could be detected in the whole cell hydrolysate. It is non chromogenic and show characteristics most closely related to Streptomyces lipmanii. It has some differences in morphological, cultural and physiological characteristics from Streptomyces lipmanii. On the basis of morphological, cultural and physiological characteristics, the strain under study was determined to be a new subspecies of Streptomyces lipmanii and was given the epithet Streptomyces lipmanii-ZAG-37
Subject(s)
Streptomyces/growth & developmentABSTRACT
Streptomyces lipmanii-ZAG-37 was grown on nutrient agar medium supplemented by 2.0% starch. The growth was used for inoculating a sterile nutrient medium of pH 7.5, incubated without shaking for 6 days at 30°. Starch is the best carbon source among the tested carbohydrates, whereas potassium nitrate and potassium dibasic phosphate are the most suitable nitrogen and phosphate sources for the enzyme bio-synthesis. On the other hand nickel salts have an inhibtory action. Riboflavin stimulated the enzyme biosynthesis when different water soluble vitamins were tested. Maximum activity for the purified enzyme was recorded at 37° for 60 min. after which the activity was decreased. Amylase showed maximum activity in presence of starch followed by dextrin then glycogen. The activity of amylase was increased by 6.0 and 9.0% in prersence of strontium and calcium salts